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STUDIES ON THE PRECIPITINOGENIC RELATIONSHIPS BETWEEN DIFFERENT SEROTYPES OF M. AFIUM AND M. INTRACELLULARE *
Author(s) -
Kubin M.,
Lind A.,
Matuskova E.,
Noriin M.
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00121.x
Subject(s) - serotype , ouchterlony double immunodiffusion , antigen , strain (injury) , microbiology and biotechnology , serology , biology , immunodiffusion , virology , immune sera , antiserum , antibody , immunology , anatomy
Antigenic preparations from 4 M. avium and 20 M. intracellulare strains belonging to 11 serotypes (Schaefer) were obtained by desintegration in a cooled high‐pressure device. The material was compared with an M. avium reference antigen prepared from a culture filtrate of strain Faisan IV (MA 4) employing an immune serum corresponding to the reference antigen. Ouchterlony's double diffusion‐in‐gel technique in a micromodification was applied. The presence or absence of two common mycobacterial precipitinogens (γ and α) and two precipitinogens (d and e) earlier mainly demonstrated only in M. avium strains was determined. The common precipitinogen γ was found in all except three strains and that designated α in all of them. The precipitinogen d was detected in 10 strains representing 7 different serotypes. The precipitinogen e was found in all M. avium strains representing two serotypes and in some M. intracellulare strains belonging to three different serotypes. The serological relationships of the two species are discussed.