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A POLYSACCHARIDE ANTIGEN OF AN ANAEROBIC ORAL FILAMENTOUS MICROORGANISM (EUBACTERIUM SABURREUM) CONTAINING HEPTOSE AND o‐ACETYL AS MAIN CONSTITUENTS
Author(s) -
Hofstad Tor
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00119.x
Subject(s) - heptose , polysaccharide , eubacterium , sephadex , chromatography , microbiology and biotechnology , chemistry , trypsin , biochemistry , antigen , biology , enzyme , bacteria , genetics , mutant , gene
A serologically highly active polysaccharide has been isolated from the filamentous oral organism, strain L44. The isolation procedure entailed the digestion of whole, acetone‐dried cells with trypsin, followed by fractionation of the supernatant fluid on columns of Sephadex G‐75 and DEAE‐cellulose. Heptose and 0‐acetyl accounted for approximately 90 per cent of the purified antigen preparations. All preparations contained small amounts of protein. Lipid or hexosamine were not found.