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LOCAL IMMUNIZATION OF GUINEA PIG MAMMARY GLAND WITH STAPHYLOCOCCAL ANTIGENS
Author(s) -
McDowell Graham,
Grov Arne,
Oeding Per
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00115.x
Subject(s) - guinea pig , immunization , mammary gland , antigen , immunology , new guinea , biology , medicine , history , cancer , ethnology , breast cancer
Immunization of the mammary glands of pregnant guinea pigs with Staphylococcus aureus or isolated mucopeptide induced a local immune response that persisted throughout lactation. Precipitin arcs corresponding to IgG, IgM, albumin and a protein of β‐electrophoretic mobility were seen in imrnunoelectrophoretic plates of whey developed using antiserum to guinea pig serum. When antiserum to guinea pig immunoglobulins was used, a strong arc in the β position and a weak IgG arc appeared. The protein of β‐electrophoretic mobility was the only component, in whey from immunized glands, which reacted with polysaccharide A (teichoic acid) in immunoelectrophoresis. No reaction with this antigen was observed for whey from non‐immunized glands or serum. It is suggested that the protein of β‐electropho‐retic mobility detected in guinea pig whey is analogous to secretory IgA from humans and other species.