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UTILIZATION OF THYMINE, THYMIDINE AND TMP BY NEISSERIA MENINGITIDIS
Author(s) -
Jyssum Sidsei.
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00111.x
Subject(s) - thymidine , thymine , thymidine phosphorylase , thymidine kinase , neisseria meningitidis , nucleoside , neisseriaceae , labelling , incubation , dna , biochemistry , enzyme , chemistry , deoxyadenosine , biology , virology , bacteria , genetics , antibiotics , virus , herpes simplex virus
Activities corresponding to thymidine phosphorylase and nucleoside deoxyribosyl transferase could not be found in Neisseria meningitidis . 14 C‐2‐thymine was bound to or taken up by whole cells, but this did not result in labelling of the TMP, TDP or TTP pools. No significant thymidine kinase activity could be induced in N. meningitidis . 14 C‐2‐thymidine which was bound to or taken up by whole cells resulted in faint labelling of the TMP, TDP and TTP pools after prolonged incubation, but no radioactive thymine appeared. The N. meningitidis extracts had activities corresponding to TMP and TDP kinases, but whole cells which were exposed to TMP‐methyl‐ 3 H did not seem to take up significant amounts of this compound. The findings indicate that N. meningitidis lacks the enzyme systems that are known to mediate a specific incorporation of thymine or thymidine into DNA.