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A CONTINUOUS PROCESS FOR THE PRODUCTION OF EXTRACELLULAR PROTEINS BY STAPHYLOCOCCUS AUREUS
Author(s) -
Holme Tord,
Wadström Torkel
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00081.x
Subject(s) - staphylokinase , extracellular , lipase , fermentation , protease , biochemistry , chemistry , microbiology and biotechnology , staphylococcus aureus , food science , chromatography , biology , enzyme , bacteria , recombinant dna , genetics , gene
A continuous process has been developed for the production of staphylococcal enzymes and toxins. In order to obtain sufficient quantities of these extracellular proteins, 40 1 volumes of culture were produced by a short‐term continuous process in a stirred fermentor with a working volume of 2.2 1. Three strains of Staphylococcus aureus , selected for high capacity in producing various enzymes and toxins, were used. Yeast extract was required as a medium component for optimal yields of the extracellular proteins. Active aeration, pH control, and prevention of foaming were ajso essential for high yields. In a series of cultivations the following growth conditions were selected: dilution rate 0.5 h ‐1 , pH 6.8, air flow 0.5 1 per min, and impeller speed 800 rpm. Each run was terminated after approximately 36 h. Constant levels were obtained after 4 h of the continuous run for bacterial dry weight, α‐toxin, leucocidin, DNase, phosphatase, protease, and staphylokinase. Lipase activity increased during 20 h before constant levels were obtained. Hyaluronate lyase decreased during the whole period of each experiment. The activity levels of phosphatase, protease, and staphylokinase were approximately the same as those in batch cultures, grown under the same conditions (2). The yields of a‐toxin and DNase were approximately half of those obtained in batch cultures. The average yield of hyaluronate lyase per 40 1 of culture supernatant from continuous culture was lower, and the average yield of lipase was higher than that obtained by the corresponding batch process.