Premium
PREPARATION AND CHEMICAL CHARACTERISTICS OF ENDOTOXIC LIPOPOLYSACCHARIDE FROM THREE STRAINS OF SPHAEROPHORUS NECROPHORUS
Author(s) -
Hofstad Tor,
Kristoffersen Tore
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00077.x
Subject(s) - heptose , rhamnose , chemistry , glucosamine , chromatography , galactosamine , galactose , mannose , biochemistry , lipopolysaccharide , fucose , sugar , biology , mutant , gene , endocrinology
Lipopolysaccharide (LPS) has been isolated from the supernatant fluid after ultracentri‐fugation of the water phase following phenol‐water extraction of Sphaerophorus necrophorus , strains ATCC 12290, 3328 Institut Pasteur and N167 Fievez. The purification procedure included digestion of the supernatant fluid with ribonuclease and deoxyribonuclease, gel filtration on Bio‐Gel A—1.5 m and ion exchange chromatography on DEAE‐cellulose. LPS from strain N167 contained small amounts of 2‐keto‐3‐deoxy‐octonate, and the major sugar components were heptose, galactose, glucose and glucosamine. 2‐keto‐3‐deoxy‐octonate and heptose were not present in LPS from strains 12290 and 3328. The sugar components identified were galactose, ' glucose, mannose, fucose, rhamnose, glucosamine and galacto‐samine. The endotoxic potency of the preparations was low.