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PHOSPHOLIPASE ACTIVITY OF ACINETOBACTER CALCOACETICUS
Author(s) -
Lehmann Vidar
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00075.x
Subject(s) - acinetobacter calcoaceticus , phosphatidylethanolamine , chemistry , phospholipase , chromatography , phospholipase a , biochemistry , phospholipase c , yolk , phosphatidylcholine , enzyme , phospholipase a2 , biology , phospholipid , membrane , food science , acinetobacter , antibiotics
Phospholipase activity in broth culture filtrate of Acinelobacter calcoaceticus has been investigated. Phosphatidylcholine and phosphatidylethanolamine were split by a reaction in accordance with phospholipase C activity. The egg yolk turbidity reaction was enhanced by Mg ++ and inhibited by Ca +‐ and Zn ++ , the temperature coefficient (Q10°C) was 1.9 and optimum pH 8‐8.4. Similarities between the haemolytic reaction and the egg yolk reaction of A. calcoaceticus were pointed out, and confirmed by gel filtration and electrophoresis experiments. Evidence suggests that the haemolytic activity of A. calcoaceticus is due to the action of a phospholipase C.