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PROTEOLYTIC ENZYMES AND BIOLOGICAL INHIBITORS
Author(s) -
Fossum Kåre
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00041.x
Subject(s) - antibody , biology , microbiology and biotechnology , inoculation , casein , proteolytic enzymes , bacteria , corynebacterium , enzyme , virology , immunology , biochemistry , genetics
Rabbits and pigs, artificially infected with washed cells of Corynebacterium pyogenes , were shown to produce inhibitory antibodies against the bacterial proteinase, as tested by electrophoresis of the sera, combined with the casein precipitating inhibition test (electrophoretic CPI‐test). The antibodies were usually detectable about 14 days after inoculation. Sera of animals not inoculated with microorganisms were also examined. In sera collected from 10 randomly selected animals of each of the species man, horse, chicken, rabbit, and dog, antibodies against Corynebacterium pyogenes proteinase could not be demonstrated. On the other hand, six, four, and two sera, out of ten, from cattle, pig, and sheep respectively, were found to contain such antiproteinases. In the sera from an additional 25 selected pigs, antibodies were demonstrated in 16; most of these pigs were shown to have chronic corynebacterial infection. Antibodies against Corynebacterium pyogenes proteinase were demonstrated in most of the sera from 34 selected cattle of different ages, especially from the older ones. In some of these animals the bacterium was isolated from pyogenic processes; in other animals there were strong indications of a corynebacterial infection, but in other animals there was no direct indication of such an infection, although previously experienced infections, with or without clinical manifestations are also supposed to have occurred in these cases. The diagnostic value of qualitative or quantitative determination of such antiproteinases in cattle and pig is considered to be of limited value, due to the frequent occurrence of such antibodies.