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IMPROVED TECHNIQUE FOR SEPARATION AND ANALYSIS OF SOLUBLE IMMUNE COMPLEXES
Author(s) -
Steensgaard J.,
Funding L.
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb00028.x
Subject(s) - separation (statistics) , sedimentation , ultracentrifuge , chromatography , analytical ultracentrifugation , chemistry , rotor (electric) , separation method , analytical chemistry (journal) , materials science , mathematics , physics , geology , statistics , paleontology , quantum mechanics , sediment
An improved technique for separation and analysis of soluble immune complexes by ratezonal ultracentrifugation is described. The separation is achieved with a B‐XIV titanium zonal rotor and a 3 to 23 per cent (w/w) optimized isokinetic sucrose gradient. This method allows an accurate calculation of sedimentation coefficients for separated complexes.