Premium
SURFACE IMMUNOGENS OF MYCOBACTERIUM TUBERCULOSIS DEMONSTRATED BY IMMUNOFLUORESCENCE
Author(s) -
Bennedsen J.
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04360.x
Subject(s) - mycobacterium tuberculosis , immunofluorescence , antigen , titer , chemistry , polyacrylamide gel electrophoresis , bacilli , tuberculosis , chromatography , electrophoresis , microbiology and biotechnology , antibody , enzyme , biology , biochemistry , bacteria , immunology , medicine , pathology , genetics
Under mild conditions, saline extraction of tubercle bacilli produced an immunogenic mixture which, in rabbits, gave rise to precipitating sera with high fluorescence antibody (FA) titre. Some purification of the major antigens was achieved by means of ion‐exchange chromatography and disc electrophoresis in polyacrylamide. At least two immunogenic substances were found, each with a different type of immunospecificity. The one gave sera that stained M. tuberculosis and BCG exclusively and, by means of enzymatic digestion, this was shown to be of protein nature.