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DEMONSTRATION OF FRANCISELLA TULARENSIS (SYN. PASTEURELLA TULARENSIS ) IN SYLVAN ANIMALS WITH THE AID OF FLUORESCENT ANTIBODIES
Author(s) -
Karlsson K.A.,
Dahlstrand S.,
Hanko E.,
Söderlind O.
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04351.x
Subject(s) - francisella tularensis , inoculation , direct fluorescent antibody , veterinary medicine , biology , microbiology and biotechnology , fluorescein isothiocyanate , antibody , medicine , fluorescence , immunology , virulence , biochemistry , gene , physics , quantum mechanics
Samples of 229 sylvan animals were investigated for tularaemia both by the FA‐test as well as by histopathological and/or conventional bacteriological investigations. For the FA‐test we used fluorescein isothiocyanate labelled anti‐F. tularensis globulin from a man who had just recovered from tularaemia. In 124 cases where all three methods were used, there was complete agreement in 85 cases (68 per cent). In 34 cases (27 per cent), the bacteriological investigations were negative whereas the other two methods gave positive results. In 105 suspected cases the FA‐technique was compared with histopathological procedures only. The results were in agreement in 103 cases. In 146 guinea pigs which had been inoculated with material from suspected cases, the results of cultivation and FA‐procedures were in agreement in 136 cases (93 per cent). In experimentally infected rabbits, tularaemia bacteria could be detected in the liver even after storage at room temperature for 25 days post mortem . The present results indicate that the FA‐technique for the detection of tularaemia in field material is a diagnostic method considerable more reliable than inoculation into guinea pigs. Furthermore, the FA‐technique is more rapid and eliminates to an essential degree the risk of laboratory infections.