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RABBIT ANTI RAT LYMPHOCYTE SERUM: CHARACTERIZATION OF THE IN VITRO LYMPHOCYTOTOXIC ANTIBODY WITH REFERENCE TO THE IMMUNIZATION PROCEDURE AND THE ANTIGEN DOSE
Author(s) -
JAKOBSEN ARNT
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04344.x
Subject(s) - antigen , immunization , antibody , antiserum , in vitro , cytotoxic t cell , immunology , booster dose , adjuvant , spleen , lymphocyte , chemistry , medicine , biochemistry
The immunoglobulin classes of the in vitro active lymphocytotoxic antibodies of different antilymphocyte sera have been investigated. Rabbits were immunized intravenously with different numbers of rat spleen cells, biweekly for 1–4 weeks. The effect of the antigen dose and the length of the immunization course was investigated. Lymphocytotoxic antibodies were found both in the IgG and in the IgM fraction of the antisera. The relative contribution of the cytotoxic activity of the IgG and the IgM fraction towards the final cytotoxic activity of the crude antiserum depended on the antigen dose and the length of the immunization course. Rabbits immunized with the antigen mixed with Freund's adjuvant intramuscularly and given a booster dose intravenously showed the same pattern as rabbits immunized intravenously.