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A PARAFFIN EMBEDDING METHOD IN KIDNEY IMMUNOFLUORESCENT STUDIES
Author(s) -
HERON IVER
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04326.x
Subject(s) - microtome , immunofluorescence , kidney , pathology , antibody , chemistry , materials science , biology , medicine , immunology , endocrinology
Immunofluorescence microscopy was carried out on kidney biopsies fixed in cold ethanol, embedded in paraffin, and sectioned by a standard microtome. Immunoglobulins could be demonstrated with glomerular localization in cases of kidney diseases in a pattern similar to that described by others who have investigated frozen specimens. β1A/β1C complement was shown to be preserved less satisfactorily by the method. The advantages of the method and precautions to be taken are discussed.