Premium
ENDOGENOUS INCORPORATION OF 32 P IN NEISSERIA MENINGITIDIS
Author(s) -
Jyssum Sidsel,
Jyssum Kaare
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04313.x
Subject(s) - succinate dehydrogenase , malonate , propionate , succinic acid , chemistry , butyrate , oxidative phosphorylation , neisseria meningitidis , fluoride , electron transport chain , biochemistry , medicinal chemistry , enzyme , inorganic chemistry , biology , bacteria , fermentation , genetics
The incorporation of 32 P which is mediated by meningococcal extracts in the presence of ADP and Mg++ is strongly increased by acetate, succinate, propionate and acetoacetate. The incorporation is due to an increased appearance of 32 P, first in ATP and later in ADP. The incorporation is dependent upon the presence of CoA, and it is inhibited by fluoride as well as of p‐chloromercuribenzoate. The incorporation does not seem to be related to an electron flux to ferricytochrome c. The acetate added is not metabolized, and the succinate effect is not reduced when the succinic dehydrogenase is inhibited, either by the addition of malonate, or by an interruption of the electron transport. The nature of the 32 P incorporation has been discussed, and the importance of the phenomenon as a source of error in experiments aimed at the study of oxidative phosphorylation has been emphasized.