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STUDIES ON THE COMPLEMENT FIXATION TEST WITH MYCOPLASMA PNEUMONIAE ANTIGEN:
Author(s) -
Eng Jan
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04274.x
Subject(s) - complement fixation test , microbiology and biotechnology , mycoplasma pneumoniae , incubation , streptococcus pneumoniae , antigen , chromatography , chemistry , biology , antibody , immunology , biochemistry , antibiotics , medicine , serology , pneumonia
The time pattern for the early development of not cell‐bound complement fixing antigen (NCBA) of M. pneumoniae , Bård strain, in filtrates from broth cultures has been examined. A filter disc technique for the preparation of washed cell inoculates which are free of NCBA and have good cell viability is described. Using this technique, NCBA could be demonstrated significantly earlier than the culmination of the number of CFU/ml, i.e. during the late growth phase of the cultures. No NCBA could be traced during the first days of incubation. The precipitability of NCBA with ammonium sulphate is reported, and a preliminary precipitation technique has been employed in the demonstration of NCBA, together with the direct measurement technique reported earlier (3, 4). The sensitivity of the former method proved to be higher than that of the latter one. It is also reported that growth of M. pneumoniae colonies can be initiated from Millipore® 0.22 μ filter discs that have been used for filtration of broth cultures of the organism. It is suggested that the significance of this fact in the isolation of M. pneumoniae from fluid materials should be investigated.