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HOST‐CONTROLLED MODIFICATION AND RESTRICTION OF FOREIGN CHROMOSOMAL AND PLASMID DNA IN SHIGELLA FLEXNERI STRAINS
Author(s) -
Kétyi J.,
Ørskov F.
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1970.tb04269.x
Subject(s) - shigella flexneri , plasmid , biology , shigella , strain (injury) , microbiology and biotechnology , gene , genetics , heterologous , dna , escherichia coli , anatomy
The hsp gene (the genetic determinant for host‐controlled restriction and modification) was transferred from an E. coli K12 strain into a Sh. flexneri strain, and in reverse the same gene from a Shigella strain was introduced into E. coli K12. The presence of the heterologous hsp gene was tested by phage T5. It was demonstrated in interrupted mating experiments with the hybrids as recipients that the hsp hybrids had acquired changed recipient characters resembling those of the strains from which the hsp originated.