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GROWTH BEHAVIOUR OF HUMAN BRAIN TUMOURS IN MATRIX CULTURES IN FRESH AUTOLOGOUS SERUM
Author(s) -
Holmström Teddy,
Saksela Eero,
Nyström Stig,
Saxén Erkki
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1970.tb03307.x
Subject(s) - pathology , matrix (chemical analysis) , thymidine , explant culture , meningioma , significant difference , biology , medicine , chemistry , in vitro , biochemistry , chromatography
Eighteen clinically benign and eleven clinically malignant human brain tumours, subdivided into four groups with five fibromatous meningiomas, seven meningotheliomatous meningiomas, six neurinomas and nine gliomas (gr. II–IV) were grown in three‐dimensional matrix cultures in fresh autologous serum. The growth and maintenance of the primary explants were measured microscopically in serial sections and by autorodiography of tritiated thymidine uptake. There was a good correlation between these two parameters, but no statistically significant difference between clinically benign and malignant tumours with respect to their growth potential in the matrix could be demonstrated. The group of clinically benign fibromatous meningiomas had a significantly higher growth potential than the other tumour groups. The morphological differentiation was well maintained in the actual explants and in many cases the outgrowing cells reproduced the original morphology within the lacunae of the matrix.