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THE APPLICATION OF A TIDAL FLOW CULTURE METHOD TO THE STUDY OF HISTOLOGICAL AND LACTATE DEHYDROGENASE (LDH)‐ISOENZYMATIC CHANGES IN PRIMARY MOUSE LUNG CELL CULTURES
Author(s) -
Langvad E.
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1970.tb02531.x
Subject(s) - explant culture , biology , oxygen tension , lactate dehydrogenase , tissue culture , oxygenation , lung , andrology , oxygen , pathology , biochemistry , medicine , enzyme , chemistry , ecology , in vitro , organic chemistry
A new “tidal flow” cultivation system, designed for the cultivation of large amounts of tissue in primary culture is described. In the present cultivation apparatus all explanted tissue shares a common gas phase and a common pool of nutritional medium where variations of pH and glucose concentration are largely kept within physiological limits throughout the duration of the cultivation. Oxygenation of the tissue is facilitated since oxygen diffusion may take place from all sides of the explants and since the tissue is only intermittedly submerged in nutritional medium. Morphologically, the degenerative changes occurring in primary explants of lung tissue are delayed. However, changes of the LDH isoenzyme pattern in favour of M‐LDH activity, which may be considered a sign of dedifferentiation at the molecular level, start immediately and are not prevented by an increased oxygen tension. It is concluded that morphological changes are preceded by changes at the molecular level.