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IN VITRO ASSAY OF THE EFFECT OF ANTIBACTERIAL TREATMENT OF THE INTESTINAL FLORA ON BONE MARROW CELL PROLIFERATION
Author(s) -
Kivilaakso E.,
Rytömaa T.,
Niskanen E.
Publication year - 1970
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1970.tb02135.x
Subject(s) - bone marrow , flora (microbiology) , in vitro , haematopoiesis , neomycin , thymidine , granulocyte , biology , microbiology and biotechnology , cell division , immunology , pharmacology , cell , antibiotics , bacteria , biochemistry , stem cell , genetics
The significance of the normal intestinal flora in maintaining the “normal” rate of granulocyte production has been investigated by studying the effect of an artificial suppression of the flora on the proliferative activity of normal bone marrow cells in vitro. The serum obtained from neomycin sulphate treated rats was shown to inhibit by 10.2 ± 1.9 per cent (P < 0.001) the incorporation of 3 H‐thymidine into the bone marrow cells as compared to the control serum. This effect was not dependent on the type of antibacterial drug used. The same inhibitory effect on the marrow cells was also demonstrated by means of radioactive precursor substances other than 3 H‐thymidine. The results have been interpreted as supporting the concept that one reason for the enormous daily production of granulocytes in “normal” conditions is the need for a protective barrier of granulocytes against the invasion of the organism by microbes and other agents from the gastrointestinal tract and elsewhere. Furthermore, the reduced resistance to systemic infections observed after an artificial suppression of the intestinal flora can perhaps be explained as a consequence of diminished granulocytopoiesis.