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ON EPITHELIAL FORMATION AFTER HOMOLOGOUS SUBCUTANEOUS TRANSPLANTATION OF URINARY BLADDER AND SKIN
Author(s) -
Jonson Gunnar
Publication year - 1956
Publication title -
acta pathologica microbiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5555
DOI - 10.1111/j.1699-0463.1956.tb03158.x
Subject(s) - trypan blue , epithelium , transplantation , urinary system , rest (music) , medicine , biology , anatomy , andrology , pathology , surgery , cell culture , genetics
SUMMARY Urinary bladders from fully developed fetuses of rabbit and slips of skin from newborn rabbits, aged 6 hours, were placed in a 1 per cent trypan blue solution from George T. Gurr (No. 06186) for about 24 hours in ordinary room temperature. They were then transferred to the hypodermis of grown‐up rabbit males. The transplants and their surroundings were microscopically examined after 14 days (the urinary‐bladders) and 21 days (the skin). The control material consisted of an identical number of transplantations of the same organ without any foregoing staining (called “direct” transfers). Healthy transitional epithelium round cysts of a varying size appeared in the trypan blue group at a frequency so markedly higher that the conclusion is drawn that trypan blue treatment causes a prolongation of the survival time of that epithelium. Epithelium of an epidermal character appeared definitely in 4 cases of skin transfer after trypan blue treatment, but in no instance among the controls (18 transfers in each group). It is stated as probable that the epithelium that was found had been formed through proliferation from regenerative centres within the transferred epithelium, while the epithelium otherwise has been lost. Possibly, the trypan blue treatment, it is also suggested, has increased the vitality in the regenerative centres. Before work on the above lines is continued, it is, however, indispensable to try to find the answers to some other questions regarding the mechanism of the trypan blue treatment, as propounded in the introduction.