Premium
Analysis of Gene Expression Profile Induced by Water Stress in Upland Rice ( Oryza sativa L. var. IRAT109) Seedlings using Subtractive Expressed Sequence Tags Library
Author(s) -
Wang Haiguang,
Zhang Hongliang,
Li Zichao
Publication year - 2007
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1672-9072.2007.00553.x
Subject(s) - suppression subtractive hybridization , biology , expressed sequence tag , oryza sativa , gene , cdna library , complementary dna , gene expression , microbiology and biotechnology , genetics
To identify the water stress induced genes of upland rice cultivar IRAT109, which is resistant to drought, a subtractive cDNA library was developed from polyethylene glycol‐ (PEG) treated and non‐treated seedlings by suppression subtractive hybridization, from which 2 112 recombinant colonies were obtained. Eight hundred clones were selected randomly for sequencing analysis, and 384 unique expressed sequence tags (ESTs) were obtained. They were found to be involved in diverse biological processes, such as metabolism, transcription, signal transduction, protein synthesis and others. Notably a number of known functional genes in drought tolerance, including genes related to biosynthesis of osmoprotectants, defense against active oxygen, removal of toxic compounds, recovery of proteins and reinforcement of cell wall were also found in the study. Several genes related to deleterious responses were upregulated by PEG stress. The differential expression patterns of 11 SSH‐derived ESTs were confirmed by real‐time polymerase chain reaction.