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A Putative Calcium‐Permeable Cyclic Nucleotide‐Gated Channel, CNGC18, Regulates Polarized Pollen Tube Growth
Author(s) -
Chang Fang,
Yan An,
Zhao LiNa,
Wu WeiHua,
Yang Zhenbiao
Publication year - 2007
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1672-9072.2007.00524.x
Subject(s) - pollen tube , tip growth , biophysics , extracellular , microbiology and biotechnology , pollen , mutant , calcium , depolarization , chemistry , biology , botany , biochemistry , gene , pollination , organic chemistry
A tip‐focused Ca 2+ gradient is tightly coupled to polarized pollen tube growth, and tip‐localized influxes of extracellular Ca 2+ are required for this process. However the molecular identity and regulation of the potential Ca 2+ channels remains elusive. The present study has implicated CNGC18 (cyclic nucleotide‐gated channel 18) in polarized pollen tube growth, because its overexpression induced wider and shorter pollen tubes. Moreover, CNGC18 overexpression induced depolarization of pollen tube growth was suppressed by lower extracellular calcium ([Ca 2+ ] ex ). CNGC18‐yellow fluorescence protein (YFP) was preferentially localized to the apparent post‐Golgi vesicles and the plasma membrane (PM) in the apex of pollen tubes. The PM localization was affected by tip‐localized ROP1 signaling. Expression of wild typeROP1 or an active form of ROP1 enhanced CNGC18‐YFP localization to the apical region of the PM, whereas expression of RopGAP1 (a ROP1 deactivator) blocked the PM localization. These results support a role for PM‐localized CNGC18 in the regulation of polarized pollen tube growth through its potential function in the modulation of calcium influxes.