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Variation in the Coat Protein Gene of Papaya ringspot Virus Isolates from Multiple Locations of China
Author(s) -
Wei JunYa,
Liu DeBing,
Li XiaoYing,
Zhou Peng
Publication year - 2007
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1672-9072.2007.00477.x
Subject(s) - papaya ringspot virus , biology , potyvirus , gene , phylogenetic tree , potyviridae , plant virus , virus , virology , population , genetics , demography , sociology
The potyvirus Papaya ringspot virus (PRSV) is an important pathogen of papaya that causes severe losses in economic crops for papaya production globally. The coat protein (CP) genes of five PRSV isolates originating from different locations in China were cloned and sequenced. The CP‐coding region varied in size from 864–873 nucleotides, encoding proteins of 288–291 amino acids. The five Chinese isolates of PRSV have been characterized as papaya‐infecting (PRSV‐P). The CP sequences of the Chinese isolates were compared with those of previously published PRSV isolates originating from different countries at amino acid levels. A number of KE repeat boxes in the N terminus of the PRSV‐CP were found in all Chinese isolates. The phylogenetic branching pattern revealed that there was certain extended grouping between geographic locations, and the Asian type probably represents the oldest population of PRSV. The information of CP genes will be useful in designing and developing durable virus resistant‐PRSV transgenic papaya in China. Meanwhile broad‐spectrum‐virus resistant, strongly resistant‐PRSV and good safe papaya lines are required.