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Actinobaculum schaalii , a cause of urinary tract infections in children?
Author(s) -
Andersen Lise Bols,
Bank Steffen,
Hertz Birgitte,
Søby Karen Marie,
Prag Jørgen
Publication year - 2012
Publication title -
acta paediatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.2011.02586.x
Subject(s) - urine , urinary system , medicine , polymerase chain reaction , incubation , incubation period , real time polymerase chain reaction , microbiology and biotechnology , biology , biochemistry , gene
Aim: To inform that Actinobaculum schaalii can colonize the urine and cause urinary tract infection in children. Methods: Urine samples were examined by wet smear microscopy, incubated in 5% CO 2 for 1–2 days, and species‐specific real‐time polymerase chain reaction (PCR) for A. schaalii was performed. Results: In 5 of the 29 screened urines, A. schaalii was found only by real‐time PCR in quantities equivalent to ≥10 4 –10 5 CFU/mL. In addition, A. schaalii was found in quantities equivalent to ≥10 6 CFU/mL by both culture and PCR in two children with a urinary tract infection and large numbers of leucocytes in the urine. Conclusion: Actinobaculum schaalii is CO 2 ‐dependent. Therefore, if there are clinical symptoms and/or a negative culture despite the presence of leucocytes in the urine, Gram staining and incubation in 5% CO 2 or species‐specific real‐time PCR should be performed to identify A. schaalii .