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CD4 + CD25 high Treg cells in peripheral blood during remission and exacerbation of allergic asthma in children
Author(s) -
Pumputiene Ingrida,
Emuzyte Regina,
Siaurys Almantas,
Tamosiunas Vytas,
Valiulis Arunas
Publication year - 2011
Publication title -
acta paediatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.2011.02241.x
Subject(s) - exacerbation , medicine , peripheral blood mononuclear cell , il 2 receptor , immunology , asthma , flow cytometry , immunoglobulin e , peripheral blood , t cell , immune system , antibody , biology , in vitro , biochemistry
Aim: To determine the percentage of CD4 + CD25 high Treg cells in peripheral blood CD4 + T cells of allergic asthmatic children during disease remission and exacerbation. Methods: Peripheral blood mononuclear cells (PBMC) and serum samples were collected from 6‐ to 11‐year‐old children with mild‐to‐moderate allergic asthma (n = 34) and from healthy controls (n = 15). CD4 + CD25 high T cells in PBMC were detected by flow cytometry. Total and specific IgE in serum were analysed by enzyme‐amplified chemiluminescence, and IL‐2 was measured by ELISA. Results: There was no significant difference in CD4 + CD25 high T‐cell proportions between asthmatic children in exacerbation and remission as compared with controls. CD4 + CD25 high T‐cell percentages were not correlated with total and specific IgE. IL‐2 was elevated in both disease remission and exacerbation but did not correlate significantly with CD4 + CD25 high T‐cell percentages. Conclusion: CD4 + CD25 high T‐cell proportion in the peripheral blood of total CD4 + T cells is not reduced in children with allergic IgE‐mediated asthma and does not differ between disease remission and exacerbation.