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Reduced IL‐10 production and ‐receptor expression in neonatal T lymphocytes
Author(s) -
Schultz C,
Strunk T,
Temming P,
Matzke N,
Härtel C
Publication year - 2007
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.2007.00375.x
Subject(s) - medicine , cd28 , immune system , receptor , cd3 , receptor expression , flow cytometry , endocrinology , immunology , interleukin 2 , t lymphocyte , t cell , cd8
Aim: To further evaluate the underlying mechanism of a formerly demonstrated immature anti‐inflammatory response in neonates (1). Methods: Interleukin (IL)‐10 production was measured by enzyme‐linked immunosorbent‐assay (ELISA) after anti‐CD3/anti‐CD28 costimulation of neonatal and adult T cells. IL‐10 receptor expression on T lymphocytes, B lymphocytes and monocytes were analysed by flow cytometry in neonates and adult controls. Results: After anti‐CD3/anti‐CD28 costimulation, IL‐10 production of neonatal T lymphocytes was profoundly reduced (median 247 pg/mL vs. 1062 pg/mL, p < 0.0001). IL‐10 receptor expression was diminished on neonatal T lymphocytes compared to adults (3% vs. 39.5% IL‐10 receptor positive lymphocytes; p < 0.0001). On neonatal B lymphocytes and monocytes the IL‐10 receptor expression was comparable to adult controls. Conclusion: The strongly reduced IL‐10 receptor expression on the main immune regulative T lymphocytes in conjunction with a significantly impaired synthesis of IL‐10 may play a crucial role in the formerly demonstrated deficient anti‐inflammatory immune response in neonates.