Premium
Genetic factors and clinical significance of acanthosis nigricans in obese Japanese children and adolescents
Author(s) -
Miura Naoko,
Ikezaki Ayako,
Iwama Saika,
Matsuoka Hisafumi,
Ito Keiko,
Sugihara Shigetaka
Publication year - 2006
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.2006.tb02202.x
Subject(s) - acanthosis nigricans , medicine , overweight , obesity , waist , endocrinology , body mass index , restriction fragment length polymorphism , insulin resistance , polymerase chain reaction , genetics , gene , biology
Aim: To clarify the clinical significance of acanthosis nigricans (AN) and the association of gene polymorphisms in the β2‐ and β3‐adrenergic receptors (B2ADR and B3ADR) in Japanese obese children and adolescents. Methods: Seventy obese subjects (56 boys, 14 girls) from 5 to 19 y of age were examined as to clinical features. Genetic analyses were performed in 83 obese subjects (61 boys, 22 girls), 2 to 17 y of age. Typing of gene polymorphisms in B2ADR and B3ADR was achieved by polymerase chain reaction (PCR) of genomic DNA and restriction fragment‐length polymorphism analysis (PCR‐RFLP). Results: The group with AN ( n =30) had higher values for percent overweight, BMI, waist circumference, fasting insulin, HOMA‐R, leptin and PAI‐1 than the AN‐negative group ( n =40), but there were no significant differences in age, sex or percent body fat between the two groups. The prevalences of B2ADR Gly16 and B3ADR Arg64 were significantly higher in AN‐positive ( n =26) than in AN‐negative ( n =57) subjects. In addition, the AN frequency was significantly higher in the group with both Gly16 and Arg64 than in the group with neither of these alleles (55.6% vs 12.5%, p < 0.05). Conclusion: We demonstrate that AN is a useful clinical marker for the severity of obesity associated with a high BMI, and that B2ADR Gly16 and B3ADR Arg64 are associated synergistically with AN in obese children and adolescents.