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Increased mucosal inflammatory cytokines in children with Helicobacter pylori‐associated gastritis
Author(s) -
Kütükçüler N,
Aydogdu S,
Göksen D,
çaglayan S,
Yagcyi RV
Publication year - 1997
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1997.tb15172.x
Subject(s) - helicobacter pylori , medicine , gastritis , gastroenterology , biopsy , gastric mucosa , spirillaceae , pathogenesis , stomach , population , tumor necrosis factor alpha , pathology , environmental health
The concentrations of tumour necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6) and IL‐1‐β in tissue homogenates of gastric mucosal biopsy specimens, and in gastric juice samples from Helicobacter pylori‐positive and ‐negative children, were determined. The study population comprised 30 children with recurrent abdominal pain attending upper gastrointestinal endoscopy. Of these patients 18 were infected with H. pylori. Cytokine concentrations in gastric biopsy homogenate supernatants and in gastric juice were measured by enzyme‐linked immunosorbent assay (ELISA). TNF‐α levels in gastric juice and in gastric biopsy homogenate supernatants in patients with H. pylori‐positive gastritis were found to be significantly higher than those in children without H. pylori infection. IL‐6 levels were also higher in H. pylori ‐infected subjects, but the difference in IL‐6 concentrations measured in gastric juice and biopsy homogenate supernatants did not reach statistical significance. IL‐1‐β concentrations in both specimens showed no significant difference between the two groups of children. It was suggested that increased levels of inflammatory cytokines, especially TNF‐α and IL‐6 generated locally within the gastric mucosa might be implicated in the pathogenesis of H. pylori‐associated gastritis in childhood.