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Photooxidant injury to glucose‐6‐phosphate dehydrogenase‐deficient erythrocytes with bilirubin as the sensitizer—an in vitro study
Author(s) -
Karthikeyan G,
Narang A,
Majumdar S,
Shivani K
Publication year - 1997
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1997.tb08899.x
Subject(s) - bilirubin , medicine , glucose 6 phosphate dehydrogenase , albumin , glucose 6 phosphate dehydrogenase deficiency , antioxidant , dehydrogenase , molar ratio , in vitro , serum albumin , endocrinology , biochemistry , enzyme , chemistry , catalysis
To evaluate the hypothesis that glucose‐6‐phosphate dehydrogenase (G6PD)‐deficient erythrocytes are more prone to photooxidant injury with bilirubin as the sensitizer, we compared the malonyl dialdehyde (MDA) levels and haematocrit values in 11 G6PD‐deficient samples with a bilirubin: albumin molar ratio of 1:2 (10mg% bilirubin, 2.5 g % albumin) photoexposed at 6 μW cm ‐2 nm ‐2 (420–460 nm), with 11 of control samples. Twelve hours after photoexposure the incremental changes in MDA levels (OD units) were higher in G6PD‐deficient samples [mean (SE) = 0.230 (0.171) vs 0.075 (0.043), p > 0.01]. Antioxidant drugs may have a protective role while G6PD‐deficient neonates undergo phototherapy.