Premium
Serum profiles of insulin‐like growth factors and their binding proteins in adults with growth hormone receptor deficiency treated with insulin like growth factor I
Author(s) -
Fielder PJ,
Gargosky SE,
Vaccarello M,
Wilson K,
Cohen P,
Diamond F,
GuevaraAguirre J,
Rosenbloom AL,
Rosenfeld RG
Publication year - 1993
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1993.tb12838.x
Subject(s) - endocrinology , medicine , radioimmunoassay , growth factor , insulin like growth factor , insulin like growth factor binding protein , receptor , growth hormone receptor , growth hormone deficiency , igfbp3 , insulin , insulin like growth factor 2 , growth hormone treatment , somatomedin , hormone , recombinant dna , growth hormone , biology , gene , biochemistry
Six adult patients with growth hormone receptor deficiency (GHRD) (2 men, 4 women) with an identical defect in the growth hormone receptor (GHR) gene, were treated with recombinant human insulin‐like growth factor I (IGF‐I), 40 μgikg S.C. twice daily, for 7 days. Serum concentrations of IGF peptide and IGF binding protein‐3 (IGFBP‐3) were measured by specific radioimmunoassays; serum IGFBPs were also measured by Western ligand blotting. The size distribution of both IGF‐I and IGF‐II was measured in serum following size‐exclusion fast‐performance liquid chromatography. IGF‐I treatment resulted in a normalization of serum IGF‐I levels on days 1–7 of treatment and a decrease in serum IGF‐II levels. The fall in IGF‐II levels and the simultaneous rise in IGF‐I levels, however, resulted in an unchanged total serum IGF level. The low IGFBP‐3 values did not significantly change during treatment, whereas there was a slight increase in IGFBP‐2 levels. Preliminary analysis of size‐fractionated sera suggested an increase in IGF‐I levels in the 40 and 150 kDa regions at the expense of IGF‐II levels. The results suggest that despite the failure of IGF‐I treatment to increase IGFBPs significantly, serum IGFBP concentrations were sufficient to maintain normal levels of IGF‐I. 0 Laron syndrome, growth hormone receptor deficiency, insulin‐like growth factors, insulin‐like growth factor binding protein