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Alterations of the Respiratory Burst of Polymorphonuclear Leukocytes from Diabetic Children A Chemiluminescence Study
Author(s) -
KANTAR A.,
WILKINS G.,
SWOBODA B.,
LITTARRU G. P.,
BERTOLI E.,
CATASSI C.,
COPPA G.,
GIORGI P. L.
Publication year - 1990
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1990.tb11508.x
Subject(s) - respiratory burst , chemiluminescence , medicine , luminol , endocrinology , basal (medicine) , diabetes mellitus , respiratory system , phorbol , pathogenesis , granulocyte , reactive oxygen species , immunology , biochemistry , chemistry , chromatography , enzyme , protein kinase c
. The respiratory burst of polymorphonuclear leukocytes was investigated in 24 children with insulin dependent diabetes mellitus and 24 healthy controls. This oxygen dependent, membrane associated process generates a number of toxic oxygen metabolites which are implicated in the pathogenesis of endothelial damage. The activity of polymorphonuclear leukocytes was studied in terms of luminol amplified chemiluminescence. It was found that the resting luminol amplified chemiluminescence activity of isolated polymorphonuclear leukocytes from diabetic children was significantly higher than that of controls (342000±174000 cpm vs. 165000±82000 cpm, p <0.01). The addition of respiratory burst inhibitors caused a significant reduction of basal chemiluminescence (> 80%). When the ratio of phorbol myristate acetate stimulated activity to basal activity was calculated and used as an activation index, it was found to be significantly reduced in diabetics relative to controls (4.29 ± 2.46 vs. 8.34±3.21, p<0.01). These observations suggest that increased release of toxic oxygen metabolites from polymorphonuclear leukocytes in diabetic subjects may play a role in the development of diabetic angiopathies.