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Bile Acid Malabsorption in Cystic Fibrosis; Membrane Vesicles, a Tool for Revealing the Role of the Ileal Brush Border Membrane
Author(s) -
ROOIJ F. W. M.,
BERG J. W. O.,
SINAASAPPEL M.,
BOSMANJACOBS E. P.,
TOUWBLOMMESTEIJN A. C.
Publication year - 1985
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1985.tb14931.x
Subject(s) - brush border , bile acid malabsorption , cystic fibrosis , bile acid , gastroenterology , ileum , taurocholic acid , vesicle , medicine , membrane , in vitro , malabsorption , in vivo , pathology , biochemistry , chemistry , biology , microbiology and biotechnology
. Increased fecal bile acid loss in cystic fibrosis (CF) may result from ileal dysfunction. A method to quantitate in vitro Na + ‐dependent taurocholate uptake into brush border membrane vesicles prepared from frozen ileum and ileal biopsy specimen is described. This transport across the ileal brush border membrane can be measured selectively, in contrast to in vivo measurements which represent a complex overall process. Preliminary results obtained with ileal specimen of 2 CF patients, suggest that in vitro bile acid uptake is low but not abnormal.