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Hematin and Bilirubin Binding to Human Serum Albumin and Newborn Serum
Author(s) -
HO C.K.,
ROBERTSON A. F.,
KARP W. B.
Publication year - 1985
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1985.tb10986.x
Subject(s) - kernicterus , albumin , bilirubin , medicine , serum albumin , plasma protein binding , hemoglobin , biochemistry , pharmacology , chemistry
. In jaundiced newborn infants, hemolytic disease is considered a risk factor for kernicterus due to the suspected competition between bilirubin and other hemoglobin breakdown products for albumin binding. We have studied the effect of hematin on bilirubin‐albumin binding using the peroxidase assay and a light‐scattering technique for measuring unbound bilirubin. Our results show that hematin does not affect bilirubin‐albumin binding. To determine if other albumin binding functions are affected by hematin, we used a microdialysis rate technique employing two ligands, diazepam and monoacetyldiaminodiphenyl sulfone (MADDS). Hematin does not utilize the diazepam binding function of albumin, but does decrease the albumin binding of MADDS. The results of this study indicate that the MADDS and bilirubin binding functions are not identical. The clinical usefulness of reserve albumin equivalent determination using MADDS is discussed.