Premium
Relation between Antibody Response to Pseudomonas aeruginosa Exoproteins and Colonization/Infection in Patients with Cystic Fibrosis
Author(s) -
GRANSTRÖM M.,
ERICSSON A.,
STRANDVIK B.,
WRETLIND B.,
PAVLOVSKIS O. R.,
BERKA R.,
VASIL M. L.
Publication year - 1984
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1984.tb17774.x
Subject(s) - elastase , pseudomonas aeruginosa , medicine , pseudomonas exotoxin , antibody , cystic fibrosis , microbiology and biotechnology , antigen , protease , exotoxin , antibiotics , immunology , enzyme , biology , bacteria , biochemistry , genetics , toxin
. Enzyme‐linked immunosorbent assay (ELISA) was used to measure the antibody response to Pseudomonas aeruginosa exotoxin A, elastase, alkaline protease and phospholipase C in patients with cystic fibrosis (CF). Only the chronically colonized patients showed elevated antibody titres to phospholipase C (22/22 patients), alkaline protease (16/22 patients), exotoxin A (15/22 patients) and elastase (5/22 patients). In a few patients where serial specimens were available, rising titres were recorded to all four antigens during periods of active infection. Antibiotic treatment resulted in decrease of titres against all four antigens, but only the anti‐exotoxin A and anti‐elastase titres decreased to normal levels. Titres to phospholipase C were the least influenced by antibiotic treatment. The results imply different roles for these exoproteins in chronic colonization versus active infection. The levels of P. aeruginosa antibodies to exoproteins could probably be used in monitoring treatment of patients with CF.