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17‐HYDROXYPROGESTERONE IN NORMAL CHILDREN AND CONGENITAL ADRENAL HYPERPLASIA Measurement in Serum by Radioimmunoassay after Thin‐layer Chromatography
Author(s) -
PETERSEN KNUD E.,
CHRISTENSEN THORKIL
Publication year - 1979
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1979.tb04990.x
Subject(s) - congenital adrenal hyperplasia , radioimmunoassay , medicine , cortisone , microgram , hydroxyprogesterone , urine , thin layer chromatography , endocrinology , hyperplasia , chromatography , steroid , hormone , in vitro , chemistry , biochemistry
Abstract. Serum 17α‐hydroxyprogesterone (17 OH‐P) was measured by a specific radioimmunoassay technique combined with thin‐layer chromatography. Normal values for children are <1.1 μg/1 (<3.3 nmol/1)—corresponding to values found in the literature. In congenital adrenal hyperplasia (CAH) values up to several hundred μg/1 are found. The values rise after ACTH stimulation and are suppressed by decadrone or cortisone treatment. The rise in 17 ketosteroids and pregnanetriol in untreated CAH is relatively smaller (15–25 fold). This clinical sensitivity of 17 OH‐P is thus valuable for the diagnosis of CAH (21 hydroxylase deficiency). Furthermore it is easier to take a blood sample than to collect urine for 24 hours. The usefulness in therapeutic monitoring is being studied.