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BIOCHEMICAL DIAGNOSIS OF CYSTINOSIS USING LEUCOCYTES
Author(s) -
WILLCOX P.,
PATRICK A. D.
Publication year - 1975
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1975.tb04390.x
Subject(s) - cystinosis , medicine , pediatrics , cystine , biochemistry , enzyme , chemistry , cysteine
. Willcox, P. and Patrick, A. D. (Institute of Child Health, London, England). Biochemical Diagnosis of Cystinosis using leucocytes. Acta Paediatr Scand 64:132, 1975. –A simple method for the biochemical diagnosis of cystinosis involves incubating leucocytes from a small volume of blood with 35 S‐cystine. Non‐protein 35 S‐IabeIled compounds are then extracted from the leucocytes and separated by thin‐layer chromatography. The excessive incorporation of 35 S‐cystine into the increased cystine pool of cystine cells is easily detected in autoradiography of the chromatograms.