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NITROBLUE‐TETRAZOLIUM REDUCTION BY NEUTROPHILS OF NEWBORN INFANTS IN IN VITRO PHAGOCYTOSIS TEST
Author(s) -
COCCHI P.,
MORI S.,
BECATTINI A.
Publication year - 1971
Publication title -
acta pædiatrica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 115
eISSN - 1651-2227
pISSN - 0803-5253
DOI - 10.1111/j.1651-2227.1971.tb06690.x
Subject(s) - nitroblue tetrazolium , phagocytosis , medicine , incubation , incubation period , pseudomonas aeruginosa , in vitro , stimulation , venous blood , immunology , microbiology and biotechnology , andrology , bacteria , biology , biochemistry , genetics
Summary Venous blood from healthy premature infants, premature infants with known bacterial infections, normal term infants and adults, was incubated in vitro with Pseudomonas aeruginosa. At 30 min intervals, blood samples were tested for the nitroblue‐tetrazolium (NBT) reducing activity of neutrophils. The results of the histochemical NBT test were compared with those of the blood controls from which bacteria were omitted. The stimulation of neutrophils with Ps. aeruginosa induces a considerable increase of NBT reduction in all 4 groups of subjects examined. The maximum rate of NBT reduction was observed after 1 hour of incubation with the test organism, and reached its lowest degree after 3 hours. By contrast the NBT reduction by un‐stimulated neutrophils of the same subjects remain unchanged during a 3 hour period of incubation.