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The function of platelet‐derived growth factor in the differentiation of mouse tongue striated muscle
Author(s) -
Suzuki E.,
Aoyama K.,
Fukui T.,
Nakamura Y.,
Yamane A.
Publication year - 2012
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/j.1601-6343.2011.01535.x
Subject(s) - myogenin , platelet derived growth factor receptor , platelet derived growth factor , growth factor , biology , myosin , endocrinology , medicine , myocyte , microbiology and biotechnology , receptor , biochemistry , myogenesis
Suzuki E., Aoyama K., Fukui T., Nakamura Y., Yamane A. The function of platelet‐derived growth factor in the differentiation of mouse tongue striated muscle.
Orthod Craniofac Res 2012; 15 :39–51. © John Wiley & Sons A/S Structured Abstract Objective – to determine the function of platelet‐derived growth factor (PDGF) in the final differentiation phase of tongue striated muscle cells. Materials and Methods – We analyzed the expressions of PDGF‐A, ‐B, platelet‐derived growth factor receptor (PDGFR)‐α, and PDGFR‐β in mouse tongues between embryonic days (E) 11 and 15. Furthermore, we examined the effects of human recombinant PDGF‐AB and the peptide antagonist for PDGFRs using an organ culture system of mouse embryonic tongue. Mouse tongues at E12 were cultured in BGJb medium containing human recombinant PDGF‐AB for 4 days or the peptide antagonist for PDGF receptors for 8 days. Results – PDGF‐A, ‐B, PDGFR‐α, and ‐β were expressed in the differentiating muscle cells between E11 and 15. The human recombinant PDGF‐AB induced increases in the mRNA expressions of myogenin and muscle creatine kinase (MCK) and the number of fast myosin heavy chain (fMHC)‐positive cells, markers for the differentiation of muscle cells. On the other hand, the peptide antagonist for PDGFRs induced suppressions in the mRNA expressions of myogenin and MCK, and the number of fMHC‐positive cells. Both the PDGF‐AB and the antagonist failed to affect the expressions of cell proliferation markers. Conclusion – These results suggest that PDGF functions as a positive regulator in the final differentiation phase of tongue muscle cells in mouse embryos.