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Histopathological changes in collagen and matrix metalloproteinase levels in articular condyle of experimental model rats with jaw deformity
Author(s) -
Watanabe A,
Yamaguchi M,
Utsunomiya T,
Yamamoto H,
Kasai K
Publication year - 2008
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/j.1601-6343.2008.00420.x
Subject(s) - condyle , matrix metalloproteinase , anatomy , medicine , matrix (chemical analysis) , matrix metalloproteinase 3 , deformity , pathology , orthodontics , surgery , chemistry , chromatography
Structured Abstract Authors – Watanabe A, Yamaguchi M, Utsunomiya T, Yamamoto H, Kasai K Objective – To investigate the dynamics of the cartilage matrix in the articular condyle after removal of a side shift plate; Emergence of type I, II, and III collagen in the matrix as well as changes in levels of matrix metalloproteinase (MMP)‐1, ‐8, and ‐13 that degrade collagen were studied histopathologically and immunohistochemically. Design – Lateral displacement of the mandible was achieved by attaching a side shift plate to the anterior teeth of the maxilla in male rats at 6 weeks. The wearing period of the side shift plate was 8 weeks. Observations were made at 0, 1, 2, 4 and 8 weeks after removal. Results – In histopathological findings, the timing of proliferation of the layer of hypertrophy varied between the bilateral sides. In immunohistochemical findings a significant decline in the expression of type II collagen in the displacement side was observed immediately after removal. Moreover, the expressions of MMPs were elevated in both sides on 0 weeks. At 1 week after removal, a significant elevated in the expression of type II collagen, MMPs was decline in both sides. Conclusions – After removal, the levels of MMP‐1, ‐8, and 13 were reduced and the emergence of type II collagen increased. Thus, cellular outgrowth was initiated to trigger intracartilaginous ossification to restore the cartilage matrix.