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Effect of chlorhexidine mouth rinse on Streptococci counts of tooth‐tissue‐borne palatal expander biofilm
Author(s) -
Maruo IT,
Rosa EAR,
Maruo H,
Tanaka O,
Guariza Filho O,
Ignácio SA,
Camargo ES
Publication year - 2008
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/j.1601-6343.2007.00418.x
Subject(s) - biofilm , chlorhexidine , serial dilution , colony forming unit , agar , microbiology and biotechnology , dentistry , streptococcus mitis , population , antimicrobial , mouth rinse , chemistry , medicine , streptococcus , biology , bacteria , pathology , genetics , alternative medicine , environmental health
Structured Abstract Authors – Maruo IT, Rosa EAR, Maruo H, Tanaka O, Guariza Filho O, Ignácio SA, Camargo ES Objectives – To assess total Streptococci (TS) counts and biofilm mass over tooth‐tissue‐borne palatal expander (TTBPE), as well as the effect of chlorhexidine (CHX) mouth rinse on these variables. Design – A cross‐sectional study design employed clinical procedures and laboratorial techniques. Setting and Sample Population – Patients who had TTBPE removal indicated were divided into two groups: a CHX group (n = 26) in which three times a day of 0.2% CHX digluconate mouth rinses were prescribed 7 days before TTBPE removal; and a control (CON) group (n = 25) in which no antimicrobial treatment was applied. Experimental Variable – ‘Gender’, ‘Age’, and ‘TTBPE wear time’ were recorded. After TTBPE removal, biofilm mass was determined by the difference between (TTBPE + biofilm) and (TTBPE only) masses. TS counts were determined by biofilm suspension followed by progressive dilutions and culture on Mitis Salivarius agar with incubation at 37°C for 72 h. Outcome Measure – Biofilm mass (mg) and Colony Forming Units of TS / mg of biofilm (CFU‐TS / mg) were calculated. Results – Total Streptococci mean values in CHX (6.77 × 10 6 CFU‐TS / mg) were statistically lower ( p < 0.01) than those in CON (3.82 × 10 7 CFU‐TS / mg), but there was no statistical difference ( p > 0.05) between CHX (168.88 mg) and CON (182.04 mg) masses nor statistical correlation ( p > 0.05) between biofilm mass and CFU‐TS / mg in the two groups. Conclusion – Chlorhexidine reduces the TS counts in TTBPE, but has no effect on biofilm mass.