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Correlation of Hsp110 expression with caspase‐3 and ‐9 during apoptosis induced by in vivo embryonic exposition to retinoic acid or irradiation in early mouse craniofacial development
Author(s) -
Gashegu J,
Vanmuylder N,
Philippson C,
ChoaDuterre M,
Rooze M,
Louryan S
Publication year - 2006
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/j.1601-6343.2006.00361.x
Subject(s) - retinoic acid , apoptosis , caspase 3 , biology , embryonic stem cell , programmed cell death , tunel assay , caspase 8 , andrology , caspase , neural crest , microbiology and biotechnology , embryogenesis , embryo , cell culture , biochemistry , medicine , genetics , gene
Structured Abstract Authors – Gashegu J, Vanmuylder N, Philippson C, Choa‐Duterre M, Rooze M, Louryan S Objective – To analyze the expression and role of three proteins (HSP110, caspase‐3 and caspase‐9) during craniofacial development. Design – Seven pregnant C57Bl/6J mice received, by force‐feeding at gestation day 9 (E9), 80 mg/kg of all‐trans retinoic acid mixed to sesame oil. Seven pregnant NMRI mice received two grays irradiation at the same gestation day. Control mice of both strains (seven mice for each strain) were not submitted to any treatment. Embryos were obtained at various stages after exposition (3, 6, 12 and 24 h), fixed, dehydrated and embedded. Coronal sections (5 μ m) were made. Slide staining occurred alternatively using anti‐Hsp110, anti‐caspase‐3 and anti‐caspase‐9 immunohistochemistry. Results – Expression of HSP110, caspase‐3 and caspase‐9 was found in cells of well‐known locations of programmed cell death. After retinoic acid exposure, expressions were increased especially in neural crest cells of mandibular and hyoid arches. Quantification of positive cells shows that caspase‐9 and Hsp110 were expressed before caspase‐3. After irradiation, the expression of the three proteins quickly increased with a maximum 3 h after irradiation. For all three models of apoptosis (physiological, retinoic‐induced and irradiation‐induced) HSP110 positive cells were more numerous than caspase‐3 positive cells. Caspase‐3 positive cells were more numerous than caspase‐9 positive cells especially in mesectodermal irradiation‐induced apoptotic cells. Conclusion – The findings show a potential function of HSP110 in apoptosis during embryo development. Caspase‐3‐expressing cells are more numerous than cells expressing caspase‐9, especially irradiation‐induced apoptotic neural crest cells. This suggests that other caspases, still to be identified, may activate caspase‐3 in this model.