Open AccessMolecular mapping of stripe rust resistance gene YrSN104 in Chinese wheat line Shaannong 104
Author(s) -
Asad Muhammad Azeem,
Xia Xianchun,
Wang Chengshe,
He Zhonghu
Publication year - 2012
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2012.02261.x
Subject(s) - biology , stripe rust , resistance (ecology) , gene , genetics , line (geometry) , rust (programming language) , plant disease resistance , agronomy , programming language , geometry , mathematics , computer science
Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a serious yield‐limiting factor for wheat production worldwide. The objective of this study was to identify and map a stripe rust resistance gene in wheat line Shaannong 104 using SSR markers. F 1 , F 2 and F 3 populations from Shaannong 104/Mingxian 169 were inoculated with Chinese Pst race CYR32 in a greenhouse. Shaannong 104 carried a single dominant gene, YrSN104 . Six potential polymorphic SSR markers identified in bulk segregant analysis were used to genotype F 2 and F 3 families. YrSN104 was closely linked with all six SSR markers on chromosome 1BS with genetic distances of 2.0 cM ( Xgwm18, Xgwm273, Xbarc187 ), 2.6 cM ( Xgwm11, Xbarc137 ) and 5.9 cM ( Xbarc240 ). Pedigree analysis, pathogenicity tests using 26 Pst races, haplotyping of associated markers on isogenic lines carrying known stripe rust resistance genes, and associations with markers suggested that YrSN104 was a new resistance gene or an allele at the Yr24/Yr26 locus on chromosome 1BS. Deployment of YrSN104 singly or in combination to elite genotypes could play an effective role to lessen yield losses caused by stripe rust.