Open AccessMolecular cloning and comparative analysis of a y‐type inactive HMW glutenin subunit gene from cultivated emmer wheat ( Triticum dicoccum L.)
Author(s) -
SUN MINMIN,
YAN YUEMING,
JIANG YI,
XIAO YINGHUA,
HU YINGKAO,
CAI MINHUA,
LI YAXUAN,
HSAM SAI L. K.,
ZELLER FRIEDRICH J.
Publication year - 2004
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2004.01835.x
Subject(s) - glutenin , biology , gene , genetics , locus (genetics) , coding region , sequence analysis , common wheat , genomic dna , protein subunit , chromosome
Cultivated emmer ( Triticum dicoccum , 2n=4x=28, AABB ) is closely related to bread wheat and possesses extensive allelic variations in high molecular weight glutenin subunit (HMW‐GS) composition. These alleles may be an important genetic resource for wheat quality improvement. To isolate and clone HMW‐GS genes from cultivated emmer, two pairs of allele‐specific (AS) PCR primers were designed to amplify the coding sequence of y‐type HMW‐GS genes and their upstream sequences, respectively. The results showed that single bands of strong amplification were obtained through AS‐PCR of genomic DNA from emmer. After cloning and sequencing the complete sequence of coding and 5′‐flanking regions of a y‐type subunit gene at Glu‐A1 locus was obtained. Nucleotide and deduced amino acid sequences analysis showed that this gene possessed a similar structure as the previously reported Ay gene from common wheat, and is hence designated as Ay1 d . The distinct feature of the Ay1 d gene is that its coding region contains four stop codons and its upstream region has a 85‐bp deletion in the same position of the Ay gene, which are probably responsible for the silencing of y‐type subunit genes at Glu‐A1 locus. Phylogenetic analysis of HMW glutenin subunit genes from different Triticum species and genomes were also carried out.