Open AccessVariation Between Two Near Isogenic Barley ( Houdeunz Vulgare ) Cultivars in Expression of the B Subunit of the Vacuolar ATPase in Response to Salinity
Author(s) -
Wef Wenxue,
Bilsborrow Paul,
Hooley Paul,
Fincham Daron,
Forster Brian
Publication year - 2001
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2001.00227.x
Subject(s) - biology , cultivar , salinity , gene , protein subunit , gene expression , hordeum vulgare , atpase , botany , salt (chemistry) , microbiology and biotechnology , genetics , poaceae , enzyme , biochemistry , ecology , chemistry
A gene encoding the barley vacuolar ATPase subunit B (BSVAP) was differentially expressed between two near isogenic barley cultivars, Golden Promise and Maythorpe. This gene (BSVAP) was isolated by the mRNA differential display technique (DDRT‐PCR). BSVAP was salt inducible under long‐term salinity stress in the salt sensitive cultivar Maythorpe but less so in the relatively salt tolerant Golden Promise and was more highly expressed under control conditions in Maythorpe. The physiological consequences of altered vacuolar ATPase expression are discussed in relation to the salt sensitivity of Maythorpe.