Open AccessGenome Relationships in the Genus Dasypyrum (Gramineae)
Author(s) -
Ohta Shoji,
Morishita Miki
Publication year - 2001
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2001.00101.x
Subject(s) - biology , ploidy , genome , hybrid , meiosis , genetics , pairing , chromosome , homologous chromosome , cytogenetics , genus , botany , gene , superconductivity , physics , quantum mechanics
To elucidate the genome relationships in the genus Dasypyrum and the ancestry of tetraploid D. breuiaristatum , two cytotypes of D. breuiaristatum and D. villosum were reciprocally crossed with one another. Chromosome pairing at the first metaphase of meiosis and fertility were examined in the F, hybrids and the parental plants. The mean pairing configuration and mean arm pairing frequency in D. villosum‐D. breuiaristatum (2x) hybrids were 11.12 I +1.44 II per cell and 0.107, respectively, and they were almost completely sterile. In D. breuiaristatum (4x)‐ D. breuiaristatum (2x) hybrid, up to seven trivalents were formed, and the mean pairing configuration was 3.38 I + 3.20 II + 3.74 III + 0.005 IV per cell. The mean arm pairing frequency and relative affinity calculated in that F, hybrid were 0.915 and 0.641, respectively. Seven bivalents and seven univalents were characteristically formed in D. villosum‐D. breuiaristatum (4x) hybrids. Based on the present results, we clearly concluded that the genome of diploid D. breuiaristatum is distantly related to the genome V of D. villosum , and that these two species have different basic genomes. We, therefore, proposed the symbol Vb for the haploid genome of diploid cytotype of D. breuiaristatum. Moreover, we concluded that tetraploid D. breuiaristatum is an autotetraploid with doubled sets of the genomes homologous with that of diploid D. breuiaristatum , and we proposed the genome constitution VbVb for the haploid genome set of tetraploid cytotype of D. breuiaristatum. Furthermore, from the chromosome pairing in the F 1 hybrids involving Moroccan and Greek accessions, it was suggested that complicated rearrangements of chromosome structure have occurred in tetraploid D. breuiaristatum in its natural populations across the entire distribution area.