Open AccessA new karyotype in Callicebus Torquatus (Cebidae, Primates)
Author(s) -
Barros Regina M. S.,
Pieczarka Julio C.,
Brigido Maria Do Ccarmo O.,
Muniz José Augusto P. C.,
Rodrigues Luis R. R.,
Nagamachi Cleusa Y.
Publication year - 2000
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2000.t01-1-00055.x
Subject(s) - karyotype , biology , secondary constriction , centromere , constitutive heterochromatin , zoology , chromosome , y chromosome , genetics , fluorescence in situ hybridization , autosome , heterochromatin , hylobates , x chromosome , microbiology and biotechnology , gene
We describe a new karyotype of Callicebus torquatus using conventional staining, G‐banding with Wright Stain, CBG, Ag‐NOR staining and fluorescence in situ hybridization (FISH) with human telomere probes and comparative analysis with the previously reported karyotype of C. torquatus torquatus (2n = 20). We studied a female specimen maintained in captivity at the Centro Nacional de Primatas (Para, Brazil). This titi monkey presented 2n = 22, with four large biarmed and six acrocentric autosome pairs; the X chromosome is a medium submetacentric. C‐bands were revealed at the centromeric region of all acrocentrics and X chromosome; punctual C‐bands also are visualized at the centromeric region in the large biarmed pairs. The NOR site was located at the long arm of pair 4, at the position of a conspicuous secondary constriction. Hybridization signals were detected exclusively at the terminal region of all chromosomes. The karyotype described here has one acrocentric pair more than that found in the literature and also differs by amount and distribution of constitutive heterochromatin. Our data support the notion that the torquatus group may be composed of distinct species, each with its own karyotype.