Open AccessChromosome Analysis and rDNA FISH in The Stag Beetle Dorcus Parallelipipedus L. (Coleoptera: Scarabaeoidea: Lucanidae)
Author(s) -
Colomba M. S.,
Vitturi R.,
Zunino M.
Publication year - 2001
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2000.00249.x
Subject(s) - biology , giemsa stain , scarabaeoidea , nucleolus organizer region , chromosome , metaphase , silver stain , genetics , zoology , heterochromatin , evolutionary biology , microbiology and biotechnology , gene
In the present work the chromosome complement (2n = 18; 8AA + XY) of the stag beetle Dorcus parallelipipedus L. (Scarabaeoidea: Lucanidae) is analyzed using conventional Giemsa staining, banding techniques and ribosomal fluorescent in situ hybridization (rDNA FISH). rDNA FISH remains the unique tool for providing a clear‐cut identification of Nucleolar Organizer Regions (NORs) when conventional banding methods such as silver‐ and CMA 3 ‐staining proved to be inadequate. The dull, homogeneous CMA 3 fluorescence of all chromosomes indicates the absence of markedly GC rich compartmentalized regions in D. parallelipipedus genome. Silver impregnation inadequacy in detecting NOR regions is to be sought in the unusual extensive silver stainability of heterochromatic material which, on the contrary of what stated for vertebrates, seems to be a common feature in Scarabaeoidea species.