Open AccessA Molecular Genetic Analysis of Eragrostis Tef (Zucc.) Trotter:non‐Coding Regions of Chloroplast DNA, 18S rDNA and the Transcription Factor VP1
Author(s) -
Espelund Mari,
Bekele Endashaw,
HolstJensen Arne,
Jakobsen Kjetill S,
Nordal Inger
Publication year - 2000
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2000.00193.x
Subject(s) - biology , eragrostis , genetics , chloroplast dna , monophyly , cladogram , intron , phylogenetic tree , coding region , dna sequencing , phylogenetics , gene , botany , evolutionary biology , clade
The non‐coding chloroplast DNA sequences of the trnL (UAA) intron and the trnL‐trnF (GAA) intergeneric spacer (IGS), the coding sequences of nuclear 18S rDNA , and the transcription factor Vpl of the cereal tef ( Eragrostis tef (Zucc.) Trotter) were studied. No intraspecific variation was found among the 6 studied tef varieties. However, the study displayed that Eragrostis tef has a number of unique traits compared to other grasses. Phylogenetic analyses of the chloroplast DNA gave three grass clades, joining Eragrostis with sorghum and maize in one. In the analysis of the 18S rDNA sequences, the three grass species were joined in a monophyletic trichotomy in the cladogram, in which maize is the most divergent, rice the least and tef intermediate. The Vpl is highly conserved. The Vpl phylogeny showed that the tef Vpl‐sequence is the hitherto most divergent Vpl‐sequence reported from a grass.