Open AccessCytogenetic Analysis of Epicauta Atomavia (Meloidae) and Palembus Dermestoides (Tenebrionidae) with Xy p Sex Determination System Using Standard Staining, C‐Bands, NOR and Synaptonemal Complex Microspreading Techniques
Author(s) -
Almeida Mara Cristina,
Zacaro Adilson Ariza,
Cella Doralice Maria
Publication year - 2000
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2000.00147.x
Subject(s) - biology , autosome , constitutive heterochromatin , nucleolus organizer region , bivalent (engine) , ploidy , meiosis , heterochromatin , chromosome , x chromosome , synaptonemal complex , genetics , chemistry , organic chemistry , gene , metal
The mitotic and meiotic chromosomes of the beetles Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) were analysed using standard staining, C‐banding and silver impregnation techniques. We determine the diploid and haploid chromosome numbers, the sex determination system and describe the chromosomal morphology, the C‐banding pattern and the chromosome(s) bearing NORs (nucleolar organizer regions). Both species shown 2n = 20 chromosomes, the chromosomal meioformula 9 + Xy p , and regular chromosome segregation during anaphases I and II. The chromosomes of E. atomaria are basically metacentric or submetacentric and P. dermestoides chromosomes are submetacentric or subtelocentric. In both beetles the constitutive heterochromatin is located in the pericentromeric region in all autosomes and in the X p chromosome; additional C‐bands were observed in telomeric region of the short arm in some autosomes in P. dermestoides. The y p chromosome did not show typical C‐bands in these species. As for the synaptonemal complex, the nucleolar material is associated to the 7th bivalent in E. atomaria and 3rd and 7th bivalents in P. dermestoides. Strong silver impregnated material was observed in association with Xy p in light and electron microscopy preparations in these species and this material was interpreted to be related to nucleolar material.