Open AccessThe Chloroplast Genomes of Azuki Bean and its Close Relatives: a Deletion Mutation Found in Weed Azuki Bean
Author(s) -
Kato Sumie,
Yamaguchi Hirofumi,
Shimamoto Yoshiya,
Mikami Tetsuo
Publication year - 2000
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2000.00043.x
Subject(s) - biology , vigna , phaseolus , genetics , chloroplast dna , radiata , botany , genus , genome , gene
A physical map of the azuki bean ( Vigna angularis cv. Erimo‐shozu) chloroplast DNA (cpDNA) was constructed by localising the cleavage sites of P stl, Sal I, Sma I, Sac I, Kpn I, Pvu I and Xho I. The resulting map is more similar to the cpDNA‐maps of two Vigna species (mung bean, V. radiata , and V. nakashimae ) than to common bean ( Phaseolus vulgaris ) cpDNA map. Azuki bean was originally classified in the genus Phaseolus , and the inclusion of this taxon in the genus Vigna is a recent taxonomic decision. Our result is thus in favour of the taxonomic placement of azuki bean in the same genus as V. nakashimae and mung bean. We also found that a weed‐form accession of azuki bean has a 96‐bp deletion relative to the cultivar Erimo‐shozu. The 96‐bp deletion is located between the trn S‐UGA and psb C genes in the large single‐copy region of the chloroplast genome. This deletion is flanked by imperfect 9‐bp direct repeats, suggesting that the deletion was a result of intra‐molecular recombination mediated by these direct repeats.